Optimization of anion exchange purification for the large-scale production of plasmid DNA for gene therapy and DNA vaccine applications

Cell & Gene Therapy Insights 2022; 8(1), 51;


Published: 15 February 2022
Jenny England

Watch the video or read the poster to learn:

  • Considerations for pDNA purification
  • How to prepare and implement a DoE approach to identify optimal conditions for various chromatography media
  • How to optimize recovery and impurity removal in the pDNA capture step
  • How to achieve a scalable and flexible pDNA purification process

Dr Jenny England is a Staff Scientist in the Applications and Innovation group in Purification and Pharma Analytics at Thermo Fisher Scientific. Jenny is a biophysicist by training and earned her PhD from Georgetown University. After graduate school, she did a post doc at the National Cancer Institute that focused on structure-based drug design for protein kinase complexes. Jenny currently leads the application group for process development of the POROS resin products for antibody, mRNA, plasmid, and viral vector purification. Additionally, Jenny evaluates new and emerging technologies that can be applied to solve unmet customer needs in the bioproduction workflow.