Rapid microbiological methods (RMMs) are essential for state-of-the-art manufacturing efficiency of cell and gene therapies because traditional testing methods do not provide shorter testing timelines and lower sample volume utilization. Some cell and gene therapies involve a manufacturing process and release timeline of just a few days making third-party testing impractical. There are ways to incorporate RMMs for QC testing, such as using a nucleic acid amplification technique as an alternative mycoplasma testing system after appropriate product-specific validation. Uncertainty around supplier selection, method validation, and costs associated with RMM implementation impede cell and gene therapy manufacturers from adopting these RMMs for QC testing. This webinar provide insight on the related issues around implementing a rapid mycoplasma PCR QC test in cell and gene therapy manufacturing to help push innovative rapid microbiological methods forward in a field that uniquely needs them.