May
5
2020
On demand

Stable AAV producer cell lines: elevating vector manufacturing

Recombinant adeno-associated virus (rAAV) has shown great promise as a suitable vector for in vivo gene therapies. Consequently, there is a rapidly increasing demand for rAAV production and robust and scalable manufacturing processes.

Currently established rAAV production methods rely on transient transfection of plasmid DNA or transduction of the producing cell with helper viruses.

Using an innovative approach based on its proprietary serum-free suspension cell line, CEVEC has developed a production system that uses rAAV producer cells, which have stably integrated into their genome all the elements required for rAAV assembly as well as the ITR-flanked transgene. For rAAV production, no further transfection steps or helper viruses are required.

The platform can be adapted to various serotypes and transgenes and has been designed for scalable production in stirred tank bioreactors. Data from production runs at different scales will be presented.

Attendees find out how to:

  • Address the production gap for AAV gene therapy vectors
  • Deliver consistent quality across batches of AAV vector through removal of the need for a transfection step
  • Achieve reliable AAV production upon induction of producer cells
  • Optimize scale-up plasmid- and helper virus-free production of AAV vectors in stirred tank bioreactors

Silke Wissing
Chief Scientific Officer

SPEAKERS

Silke Wissing
Chief Scientific Officer

You might also like

Thinking translational: Things to consider in CAR T cell research workflows

S Rösch, PhD
Saskia Rösch, PhD
Saskia Rösch, PhD
Global Product Manager at Miltenyi Biotec
22 Jun 2022
36
Days
8
Hrs
48
Min
Register

Accelerating cell therapy discovery & development with non-viral gene...

D Hermanson
X Patrinostro
David Hermanson
David Hermanson
Senior Manager of R&D Applications, Cell and Gene Therapy at Bio-techne
Xiaobai Patrinostro
Xiaobai Patrinostro
Manager of Genome Engineering Services, Cell and Gene Therapy at Bio-techne
16 Jun 2022
29
Days
16
Hrs
48
Min
Register

Applying an HPLC analytical platform for mRNA process monitoring

N Pavlin
Nejc Pavlin
Nejc Pavlin
Project Manager in Process Analytics Development at BIA Separations
7 Jun 2022
21
Days
8
Hrs
48
Min
Register

Optimizing E. Coli cell growth performance with in-line, real-time OD600...

T Buch
R Shanbaky
Tanja Buch
Tanja Buch
Technical Director at Biifactory Competence Center
Ramsey  Shanbaky
Ramsey Shanbaky
Bioprocess Applications Manger at C Technologies, Inc - a Repligen Company
1 Jun 2022
14
Days
16
Hrs
48
Min
Register

Monitoring of circulating CAR T cells

A Graband,
D Hünecke,
D Bremm
Annika Graband
Annika Graband
Product Manager, Cell Analysis Reagents at Miltenyi Biotec
Dr. Sabine Hünecke
Dr. Sabine Hünecke
Head of Laboratory, Stem Cell Transplantation and Immunotherapy at University Hospital Frankfurt
Dr. Melanie Bremm
Dr. Melanie Bremm
Deputy Head of Laboratory, Stem Cell Transplantation and Immunotherapy at University Hospital Frankfurt
31 May 2022
14
Days
8
Hrs
48
Min
Register