Feb
1
2022
On demand

Accelerate process development with at-line concentration measurement

Currently, the most commonly used methods for adeno-associated viral (AAV) vector quantitative analysis are qPCR and ELISA assays, along with analytical ultracentrifugation (AUC) and transmission electron microscopy (TEM). However, the time to results when using these methods can range from days to several weeks, and the acceptable tolerance range is high. Rapid, reliable in-process testing offers a significant benefit to AAV downstream process development, and can be achieved with Slope Spectroscopy utilizing Variable Pathlength Technology (VPT).

In this webinar, we will demonstrate how the CTech SoloVPE System, when used at-line in one or more stages of a process, can help to quickly identify process characteristics, provide key insights and allow for process optimization in minutes. VPT has enabled at-line measurement of concentration by eliminating the need for sample dilution or manipulation and delivering rapid and highly accurate real-time results.

  • Introduction to Slope Spectroscopy – a different method for UV-Vis concentration measurement
  • Review the Gene Therapy workflow for potential opportunities to accelerate testing to drive process efficiencies
  • Present gene therapy case studies demonstrating the CTech SoloVPE System performance

Joe Ferraiolo

Associate Director, Bioanalytics, Repligen

Joe leads the bioanalytics applications team and is in charge of the SoloVPE variable pathlength spectroscopy system for at-line applications. He has been with the company for more than 20 years, with over ten years of development and validation experience in analytical applications. He specializes in UV analysis and leads the development and commercialization of high-value products and flexible solutions that address critical steps in the production of biologics.

SPEAKERS

Joe Ferraiolo
Associate Director, Bioanalytics at Repligen

You might also like

Accelerating cell therapy discovery & development with non-viral gene...

D Hermanson
X Patrinostro
David Hermanson
David Hermanson
Senior Manager of R&D Applications, Cell and Gene Therapy at Bio-techne
Xiaobai Patrinostro
Xiaobai Patrinostro
Manager of Genome Engineering Services, Cell and Gene Therapy at Bio-techne
16 Jun 2022
29
Days
16
Hrs
1
Min
Register

Applying an HPLC analytical platform for mRNA process monitoring

N Pavlin
Nejc Pavlin
Nejc Pavlin
Project Manager in Process Analytics Development at BIA Separations
7 Jun 2022
21
Days
8
Hrs
1
Min
Register

Optimizing E. Coli cell growth performance with in-line, real-time OD600...

T Buch
R Shanbaky
Tanja Buch
Tanja Buch
Technical Director at Biifactory Competence Center
Ramsey  Shanbaky
Ramsey Shanbaky
Bioprocess Applications Manger at C Technologies, Inc - a Repligen Company
1 Jun 2022
14
Days
16
Hrs
1
Min
Register

Manufacturing considerations for viral and non-viral platform selection

V Slepushkin,
A Noyes,
A Hagerman et al.
Vladimir Slepushkin
Vladimir Slepushkin
Global Head of Manufacturing at MedTherapy Biotech
Aaron Noyes
Aaron Noyes
Vice President of Development at Codiak Biosciences
Allison Hagerman
Allison Hagerman
Vice President, Product Development, Oncolytics Biotech
Laurens  Sierkstra
Laurens Sierkstra
Senior Director, Business Leader, Purification at Thermo Fisher Scientific
10 May 2022
Watch now

From cells to purified capsids: How to develop a scalable rAAV process

Å Hagner McWhirter
Åsa Hagner McWhirter
Åsa Hagner McWhirter
Principal Scientist at Cytiva
3 May 2022
Watch now