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Lentiviral vectors (LVV) are of increasing interest in cell and gene therapy, but high costs and variable vector productions continue to limit patient access. LVV packaging and producer cell lines that yield titers as high as the four-plasmid method will reduce cost and process variability, as they rely less on plasmid transfection for production of LVV.
In this webinar, we will explore the initial development of an LVV packaging cell line by random integration of Tet-regulated VSV-G and Gag-Pol, and constitutive Rev (codon optimized and regions of DNA homology deleted to decrease risk of recombination) into clonal WXATUS0028 suspension HEK 293 cell line, followed by clonal isolation of the top performing clone. Subsequently, we developed an LVV producer cell line by transposon-facilitated integration of LVV genome into our top packaging cell line clone, followed by clonal isolation of the top performing clone. We will also describe trialed production of LVV encoding several different therapeutically relevant transgenes.
Attend this webinar to find out how to: