Spectrophotometric analysis is one of the most common techniques used to quantify nucleic acids for the production of vaccines or therapeutics. More specifically, the 260/280 UV absorbance ratio of the nucleic acid can be used to determine their purity. However, traditional fixed-pathlength spectrophotometers have limitations when determining the purity ratios of these molecules. Traditional UV-Vis spectrophotometers utilize a fixed 1 cm pathlength and samples most often require sample prep and/or dilution, which ultimately can introduce unnecessary risk and potential for error.
In this webinar, we will share the steps we took to validate the CTech SoloVPE System using the Slope Spectroscopy method for DNA purity determination. The method was evaluated by assessing the specificity, intermediate precision, repeatability, linearity, and accuracy of the theoretical purity ratios. The observed purity ratios from the SoloVPE System demonstrated great comparability with the theoretical purity ratios while eliminating unnecessary risk associated with sample dilution requirements.